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新型冠状病毒研究相关的实验细胞资源推介

新冠肺炎疫情已在全球范围内持续了一年多的时间，造成全球除少数几个国家和地区以外的全部地区皆陷入了程度不一的公共卫生危机。随着国内新冠病毒肺炎疫情的持续向好，我们已经从“抗疫”转变为“防疫”状态。这些成果的取得离不开无数医务工作者和科研工作者的辛勤付出，围绕新冠病毒做了大量的分离、检定、诊断、防控等工作。为了更好地完成新型冠状病毒疫情防控和科研支撑工作，国家模式与特色实验细胞资源库总结了部分与新冠病毒研究相关的实验细胞资源，并将相关参考信息汇总如下： 

一、已被证实可用于扩增SARS-CoV-2的细胞系

1、非洲绿猴肾细胞VERO C1008 (E6)

资源链接：https://www.cellbank.org.cn/search-detail.php?id=60

参考文献：

[1]. Matsuyama, Shutoku et al. “Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.” Proceedings of the National Academy of Sciences of the United States of America vol. 117,13 (2020): 7001-7003. doi:10.1073/pnas.2002589117

[2]. Zhu, Na et al. “A Novel Coronavirus from Patients with Pneumonia in China, 2019.” The New England journal of medicine vol. 382,8 (2020): 727-733. doi:10.1056/NEJMoa2001017

[3]. Wang, Manli et al. “Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro.” Cell research vol. 30,3 (2020): 269-271. doi:10.1038/s41422-020-0282-0 

2、非洲绿猴肾细胞Vero

资源链接：https://www.cellbank.org.cn/search-detail.php?id=624

参考文献：

[1]. Matsuyama, Shutoku et al. “Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.” Proceedings of the National Academy of Sciences of the United States of America vol. 117,13 (2020): 7001-7003. doi:10.1073/pnas.2002589117

[2]. Ou, Xiuyuan et al. “Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.” Nature communications vol. 11,1 1620. 27 Mar. 2020, doi:10.1038/s41467-020-15562-9

3、人结直肠腺癌细胞Caco-2

资源链接：https://www.cellbank.org.cn/search-detail.php?id=246

参考文献：

[1]. Kim, Jeong-Min et al. “Identification of Coronavirus Isolated from a Patient in Korea with COVID-19.” Osong public health and research perspectives vol. 11,1 (2020): 3-7. doi:10.24171/j.phrp.2020.11.1.02

4、人肺腺癌细胞Calu-3

资源链接：https://www.cellbank.org.cn/search-detail.php?id=262

参考文献：

[1]. Matsuyama, Shutoku et al. “Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.” Proceedings of the National Academy of Sciences of the United States of America vol. 117,13 (2020): 7001-7003. doi:10.1073/pnas.2002589117

[2]. Ou, Xiuyuan et al. “Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.” Nature communications vol. 11,1 1620. 27 Mar. 2020, doi:10.1038/s41467-020-15562-9

5、人胚肾细胞293T

资源链接：https://www.cellbank.org.cn/search-detail.php?id=24

参考文献：

[1]. Harcourt, Jennifer et al. “Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States.” Emerging infectious diseases vol. 26,6 (2020): 1266-1273. doi:10.3201/eid2606.200516

6、人肝癌细胞Huh-7

资源链接：https://www.cellbank.org.cn/search-detail.php?id=535

参考文献：

[1]. Harcourt, Jennifer et al. “Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States.” Emerging infectious diseases vol. 26,6 (2020): 1266-1273. doi:10.3201/eid2606.200516

[2]. Zhu, Na et al. “A Novel Coronavirus from Patients with Pneumonia in China, 2019.” The New England journal of medicine vol. 382,8 (2020): 727-733. doi:10.1056/NEJMoa2001017

[3]. Ou, Xiuyuan et al. “Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.” Nature communications vol. 11,1 1620. 27 Mar. 2020, doi:10.1038/s41467-020-15562-9

7、恒河猴肾细胞LLC-MK2

资源链接：https://www.cellbank.org.cn/search-detail.php?id=50

参考文献：

[1]. Ou, Xiuyuan et al. “Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.” Nature communications vol. 11,1 1620. 27 Mar. 2020, doi:10.1038/s41467-020-15562-9

二、已被证实不适合扩增SARS-CoV-2的细胞系

1、人非小细胞肺癌细胞A549

资源链接：https://www.cellbank.org.cn/search-detail.php?id=518

参考文献：

[1]. Matsuyama, Shutoku et al. “Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.” Proceedings of the National Academy of Sciences of the United States of America vol. 117,13 (2020): 7001-7003. doi:10.1073/pnas.2002589117

[2]. Harcourt, Jennifer et al. “Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States.” Emerging infectious diseases vol. 26,6 (2020): 1266-1273. doi:10.3201/eid2606.200516

三、可用于疫苗研发的实验细胞资源

1、人慢性髓系白血病细胞K-562

资源链接：https://www.cellbank.org.cn/search-detail.php?id=724

参考文献：

       [1]. Ji, Henry et al. “Novel decoy cellular vaccine strategy utilizing transgenic antigen-expressing cells as immune presenter and adjuvant in vaccine prototype against SARS-CoV-2 virus.” Medicine in drug discovery vol. 5 (2020): 100026. doi:10.1016/j.medidd.2020.100026

2、中国仓鼠卵巢细胞亚株CHO-K1

资源链接：https://www.cellbank.org.cn/search-detail.php?id=7

参考文献：

[1]. Ren, Wenlin et al. “Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primates.” Vaccine vol. 38,35 (2020): 5653-5658. doi:10.1016/j.vaccine.2020.06.066

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