中国科学院细胞库细胞株信息
细胞名称
Cell Name
3T3-Swiss albino
目录号
Serial
SCSP-5012
标识符
Identifier
CSTR:19375.09.3101MOUSCSP5012
描述
Description
此细胞株于1962年由G. Todaro 和 H. Green从分解的Swiss小鼠胚胎中建立。其生长特性为接触抑制型,长满的单层细胞量是40000个/cm2,饱和密度可以达到约50000个/cm2。该细胞在玻璃表面上可能长不好,需要长在塑料培养瓶中。检测表明鼠痘病毒(ectromelia virus,ECTV)阴性。
每个批次均通过本库支原体检测,结果为阴性。
STR鉴定结果:
①该株细胞DNA进行小鼠细胞STR分型结果显示,扩增后图谱清晰,分型结果良好:1-1:10,15;1-2:13;2-1:9;3-2:14;4-2:19.3;5-5:13,15;6-4:15.2;6-7:12,15;7-1:25.2,29;8-1:15,16;11-2:15;12-1:19;13-1:15.1;15-3:20.3;17-2:12,15;18-3:17,21;19-2:12;X-1:26。
②该株细胞确为小鼠细胞,没有人源细胞污染。
动物种别
Organism
小鼠
性别
Gender
组织来源
Tissue and Cell Type
胚胎
形态
Morphology
成纤维细胞样,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
小鼠胚胎成纤维细胞3T3-Swiss albino完全培养液配方(100 ml):
DMEM (Invitrogen, 11960-044) 88 ml
新生牛血清(Gibco)灭活后使用 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
(备注:细胞不能过密,请于细胞融合度80%时或之前传代)
不能使用胎牛血清FBS培养该细胞!
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCE Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596 Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 Todaro GJ, et al. Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40. Virology 27: 179-185, 1965. PubMed: 4284655 Todaro GJ, et al. Transformation of properties of an established cell line by SV40 and polyoma virus. Proc. Natl. Acad. Sci. USA 51: 66-73, 1964. PubMed: 14104605 Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 Chen ST, et al. Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitiis virus by using a modified tetracycline inducible system. Proc. Natl. Acad. Sci. USA 93: 10057-10062, 1996. PubMed: 8816750 Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936
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中文名称 小鼠胚胎成纤维细胞(2016年新引进)

价格
Transmittal Fee

1600

细胞库联系方式

 
电话:021-54920405 
传真:021-54920406 
联系人:陈松华、吴军 
E-mail:ecell@sibs.ac.cn 
地址:中国科学院细胞库,徐汇区岳阳路320号,上海200031

干细胞库联系方式

  
电话:021-54921358 
传真:021-54921358 
联系人:顾婷玉 
E-mail:scell@sibcb.ac.cn 
地址:中国科学院细胞库,徐汇区岳阳路320号,上海200031

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