细胞名称 Cell Name |
3T3-Swiss albino |
目录号 Serial |
SCSP-5012 |
标识符 Identifier |
CSTR:19375.09.3101MOUSCSP5012 |
描述 Description |
此细胞株于1962年由G. Todaro 和 H. Green从分解的Swiss小鼠胚胎中建立。其生长特性为接触抑制型,长满的单层细胞量是40000个/cm2,饱和密度可以达到约50000个/cm2。该细胞在玻璃表面上可能长不好,需要长在塑料培养瓶中。检测表明鼠痘病毒(ectromelia virus,ECTV)阴性。 每个批次均通过本库支原体检测,结果为阴性。 STR鉴定结果: ①该株细胞DNA进行小鼠细胞STR分型结果显示,扩增后图谱清晰,分型结果良好:1-1:10,15;1-2:13;2-1:9;3-2:14;4-2:19.3;5-5:13,15;6-4:15.2;6-7:12,15;7-1:25.2,29;8-1:15,16;11-2:15;12-1:19;13-1:15.1;15-3:20.3;17-2:12,15;18-3:17,21;19-2:12;X-1:26。 ②该株细胞确为小鼠细胞,没有人源细胞污染。 |
动物种别 Organism |
小鼠 |
性别 Gender |
|
组织来源 Tissue and Cell Type |
胚胎 |
形态 Morphology |
成纤维细胞样,贴壁生长 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
小鼠胚胎成纤维细胞3T3-Swiss albino完全培养液配方(100 ml): DMEM (Invitrogen, 11960-044) 88 ml 新生牛血清(Gibco)灭活后使用 10 ml Glutamax (Invitrogen, 35050061) 1 ml Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml (备注:细胞不能过密,请于细胞融合度80%时或之前传代) 不能使用胎牛血清FBS培养该细胞! |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
REFERENCE | Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596 Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 Todaro GJ, et al. Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40. Virology 27: 179-185, 1965. PubMed: 4284655 Todaro GJ, et al. Transformation of properties of an established cell line by SV40 and polyoma virus. Proc. Natl. Acad. Sci. USA 51: 66-73, 1964. PubMed: 14104605 Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 Chen ST, et al. Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitiis virus by using a modified tetracycline inducible system. Proc. Natl. Acad. Sci. USA 93: 10057-10062, 1996. PubMed: 8816750 Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 |
细胞说明书 | 登录后下载 |
中文名称 | 小鼠胚胎成纤维细胞(2016年新引进)(复苏包邮;或可提供冻存管) |
价格 |
1600 |
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