| 细胞名称 Cell Name |
Hep G2 [HEPG2] |
| 目录号 Serial |
SCSP-510 |
| 标识符 Identifier |
CSTR:19375.09.3101HUMSCSP510 |
| 描述 Description |
人肝癌细胞Hep G2 [HEPG2]是1975年从一名罹患肝癌的15岁白人男性青年的肿瘤组织中分离获得。据报道，该细胞不携带乙肝病毒基因组，但表达一系列蛋白，包括甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、转铁蛋白、α-1-抗凝乳蛋白酶、结合珠蛋白、铜蓝蛋白、纤溶酶原、补体C4、C3激活物、纤维蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、视黄醇结合蛋白等；表达胰岛素受体和胰岛素样生长因子IGFⅡ的受体，表达3-羟基-3-甲基戊二酰还原酶(3-hydroxy-3-methylglutaryl-CoA)和肝三酰甘油脂肪酶活性。另据资料显示，该细胞在免疫抑制小鼠中不致瘤，在半固体培养基中可成瘤，可用于大规模培养体系，已广泛应用于细胞毒理学实验和肿瘤生物学研究。 细胞质控检测（可提供检测报告）： 1) 支原体检测结果：阴性 2) 细菌检测结果：阴性 3) 真菌检测结果：阴性 4) 经STR检测结果无误。STR结果如下：D5S818: 11,12；D13S317: 9,13；D7S820: 10,10；D16S539: 12,12；vWA: 17,17；THO1: 9,9；Amelogenin: X，Y；TPOX: 8,9；CSF1PO: 10,11 |
| 动物种别 Organism |
人，白人 |
| 性别 Gender |
男性，15岁 |
| 组织来源 Tissue and Cell Type |
肝脏；疾病：肝细胞癌 |
| 形态 Morphology |
上皮样，贴壁生长；部分团块生长 |
| 培养基和添加剂 Complete Growth Medium and Culture Conditions |
人肝癌细胞 Hep G2完全培养液（目录号：SCSP-651）配方： MEM培养基 (Gibco，货号11095080)：89% 胎牛血清FBS (Gibco)：10% 非必需氨基酸溶液NEAA (Gibco，货号11140050)：1% 参考传代比例：1:3 参考传代周期：3-5天 参考换液频率：2-3天换液一次 冻存液：完全培养液95%，DMSO 5% 培养条件：气相：空气95%，二氧化碳5%；温度：37摄氏度。 细胞运输方式：活细胞保温运输或冻存管干冰运输。 【注意事项】： 1. Hep G2 [HEPG2]对培养条件要求较严格，特别是 pH 值和血清质量，否则可能影响细胞凝集数量，应使用高质量低内毒素，未灭火的胎牛血清，可以帮助圆形的细胞丛簇更好的贴壁及形成单层细胞。细胞内容易有空泡，特别是在融合时，这属于正常现象。 2. 该细胞部分团块生长，复苏后可能存在形态不典型的情况，经几次传代后，上皮细胞形态会更加典型。 3. 该细胞对血清质量较为敏感，我库建议您使用优质胎牛血清进行培养或选择订购我库配套MEM完全培养液（目录号为SCSP-651）。 |
| 供应限制 Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE | Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960 Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983 Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137 Busch SJ, et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2. J. Biol. Chem. 265: 22474-22479, 1990. PubMed: 2176219 Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851 Cuthbert C, et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed: 9169468 Deleersnyder V, et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed:8985401 Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004 Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Lewis W, et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed: 8622980 Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491 Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Lieber A, et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024 Dubuisson J, Rice CM. Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615 Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Kounas MZ, et al. Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J. Biol. Chem. 271: 6523-6529, 1996. PubMed: 8626456 Klemm DJ, et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493 Wu X, et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. PubMed: 8626595 Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983 |
| 细胞说明书 | 登录后下载 |
| 中文名称 | 人肝癌细胞（活细胞顺丰包邮；或可提供冻存管） |
|
价格 |
1200 |
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E-mail：ecell@sibs.ac.cn
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