| 细胞名称 Cell Name |
NIH/3T3 |
| 目录号 Serial |
SCSP-515 |
| 标识符 Identifier |
CSTR:19375.09.3101MOUSCSP515 |
| 描述 Description |
NIH/3T3是从小鼠NIH/Swiss品系的胚胎中分离并建立的成纤维细胞株。与其它3T3细胞的建系方法一样，NIH/3T3细胞是从NIH Swiss小鼠胚胎培养物中建立的高度接触抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了5轮以上亚克隆。该细胞易于转染，对DNA转化及转染研非常有用。据报道，该细胞对肉瘤病毒的转化灶形成和白血病病毒增殖高度敏感，鼠痘病毒（ectromelia virus，ECTV）检测阴性。 细胞质控检测（可提供检测报告）： 1) 支原体检测结果：阴性 2) 细菌检测结果：阴性 3) 真菌检测结果：阴性 4) STR鉴定结果： ①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好: 1-1: 10；1-2: 13, 17；2-1: 9；3-2: 14, 15；4-2: 19.3, 20.3；5-5: 14, 15；6-4: 15.3；6-7: 12；7-1: 29；8-1: 15；11-2: 15, 17；12-1: 20；13-1: 16.2；15-3: 20.3；17-2: 13, 14；18-3: 17, 19；19-2: 11, 12；X-1: 25。 ②该细胞与ExPASy中NIH 3T3细胞的STR数据匹配率为100%。 ③该株细胞确为小鼠细胞，没有人源细胞污染。 |
| 动物种别 Organism |
小鼠，品系NIH/Swiss |
| 性别 Gender |
|
| 组织来源 Tissue and Cell Type |
胚胎 |
| 形态 Morphology |
成纤维细胞，贴壁生长 |
| 培养基和添加剂 Complete Growth Medium and Culture Conditions |
小鼠胚胎成纤维细胞NIH/3T3 完全培养液（目录号：SCSP-646）配方： DMEM培养基 (Gibco, 货号11995065)：90% bovine calf serum，新生牛血清 (四季青，22012-8612；或Gibco，26010074)：10% 参考传代比例：1：3-1：4 参考传代周期：2~3天 参考换液频率：2~3天，传代时换液 冻存液配方：完全培养液95%，DMSO 5% 培养条件：气相：空气95%，二氧化碳5%；温度：37摄氏度。 细胞运输方式：活细胞保温运输或冻存管干冰运输。 【注意事项：】 1. 不可使NIH/3T3细胞生长过密至完全汇合（细胞将失去接触抑制的特性）。细胞密度达到80%即可传代，每周至少传代2次。 2. 该细胞对于血清较为敏感，不使用胎牛血清（FBS）培养该细胞。 |
| 供应限制 Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE | Jainchill JL, et al. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J. Virol. 4: 549-553, 1969. PubMed: 4311790 Andersson P, et al. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell 16: 63-75, 1979. PubMed:84715 Copeland NG, Cooper GM. Transfection by exogenous and endogenous murine retrovirus DNAs. Cell 16: 347-356, 1979. PubMed: 222457 Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256 Jones PL, et al. Tumor necrosis factor alpha and interleukin-1beta regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-beta and NF-kappaB. Mol. Cell. Biol. 17: 6970-6981, 1997. PubMed:9372929 Gonzalez Armas JC, et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915 Siess DC, et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675 Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Medin JA, et al. Correction in trans for Fabry disease: expression, secretion, and uptake of alpha-galactosidase A in patient-derived cells driven by a high-titer recombinant retroviral vector. Proc. Natl. Acad. Sci. USA 93: 7917-7922, 1996. PubMed: 8755577 Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Cranmer LD, et al. Identification, analysis, and evolutionary relationships of the putative murine cytomegalovirus homologs of the human cytomegalovirus UL82 (pp71) and UL83 (pp65) matrix phosphoproteins. J. Virol. 70: 7929-7939, 1996. PubMed:8892916 Shisler J, et al. Induction of susceptibility to tumor necrosis factor by E1A is dependent on binding to either p300 or p105-Rb and induction of DNA synthesis. J. Virol. 70: 68-77, 1996. PubMed:8523594 Cavanaugh VJ, et al. Murine cytomegalovirus with a deletion of genes spanning HindIII-J and -I displays altered cell and tissue tropism. J. Virol. 70: 1365-1374, 1996. PubMed: 8627652 Westerman KA, Leboulch P. Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc. Natl. Acad. Sci. USA 93: 8971-8976, 1996. PubMed: 8799138 The NIH/3T3, a continuous cell line of highly contact-inhibited cells was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 (ATCC CCL-92) and the inbred BALB/c 3T3 (ATCC CCL-163). The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. |
| 细胞说明书 | 登录后下载 |
| 中文名称 | 小鼠胚胎成纤维细胞（活细胞顺丰包邮；或可提供冻存管） |
|
价格 |
1200 |
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