| 细胞名称 Cell Name |
WPMY-1 |
| 目录号 Serial |
GNHu36 |
| 标识符 Identifier |
CSTR:19375.09.3101HUMGNHu36 |
| 描述 Description |
WPMY-1于1992年从一名54岁白人男性供者的基质中分离出来是一种肌成纤维基质细胞系， WPMY-1, 与RWPE-1 cells 一样，来源于同一张成人前列腺组织切片的周围区域的基质细胞。 通过一个pRSTV质料结构，用SV40大T抗原对基质细胞进行永生化。 WPMY-1细胞，与RWPE-1细胞及其它上皮细胞衍生株一样，属于来源于同一个前列腺的一系列细胞株。 由于它们来源于同一个前列腺的周围区域，WPMY-1细胞株对于研究分泌和基质与上皮细胞相互作用尤其有用。据提供者报道，来源于同一个前列腺的RWPE-1细胞株，经过检测，乙肝、丙肝、人免疫缺陷病毒都呈阴性。 细胞在本库通过支原体检测阴性（可供检测报告）。 细菌检测结果：阴性；真菌检测结果：阴性 细胞在本库通过STR检测无误（可供检测报告）。 STR鉴定结果： Amelogenin: X,Y D5S818:12,15 D13S317: 8,14 D7S820: 10,11 D16S539: 9 vWA: 14,18 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 13 D19S433: 13 D21S11: 29,31 D18S51: 14,16 |
| 动物种别 Organism |
人 白人 |
| 性别 Gender |
男性 |
| 组织来源 Tissue and Cell Type |
器官： 前列腺组织： 基质疾病： 正常 |
| 形态 Morphology |
成肌纤维细胞 贴壁生长 |
| 培养基和添加剂 Complete Growth Medium and Culture Conditions |
人正常前列腺基质永生化细胞WPMY-1完全培养液（目录号MD68）组分： DMEM培养基(GIBCO货号：10566016)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 培养参考：细胞消化时间2-3min。传代比例1:2，传代周期2-3天 冻存液配方：完全培养液92%，DMSO 8% 细胞运输方式：活细胞可保温装置运输或干冰冻存管。 注意事项：WPMY-1细胞生长较快，培养时细胞密度不能过高，70-80%即可传代。 |
| 供应限制 Permits and Restrictions |
A。仅供研究之用。 |
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A human prostatic stromal myofibroblast cell line WPMY-1: a model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20: 1185-1192, 1999. PubMed: 10383888 89998: Bello-DeOcampo D, et al. The role of alpha 6 beta 1 integrin and EGF in normal and malignant acinar morphogenesis of human prostatic epithelial cells. Mutat. Res. 480-481: 209-217, 2001. PubMed: 11506815 90372: Webber MM, et al. Modulation of the malignant phenotype of human prostate cancer cells by N-(4-hydroxyphenyl)retinamide (4-HPR). Clin. Exp. Metastasis 17: 255-263, 1999. PubMed: 10432011 90375: Sharp RM, et al. N-(4-hydroxyphenyl)retinamide (4-HPR) decreases neoplastic properties of human prostate cells: an agent for prevention. Mutat. Res. 496: 163-170, 2001. PubMed: 11551492 90376: Carruba G, et al. Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells. Prostate 50: 73-82, 2002. PubMed: 11816015 90377: Achanzar WE, et al. 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Chronic arsenic-exposed human prostate epithelial cells exhibit stable arsenic tolerance: mechanistic implications of altered cellular glutathione and glutathione S-transferase. Toxicol. Appl. Pharmacol. 183: 99-107, 2002. PubMed: 12387749 90385: Achanzar WE, et al. Inorganic arsenite-induced malignant transformation of human prostate epithelial cells. J. Natl. Cancer Inst. 94: 1888-1891, 2002. PubMed: 12488483 186709: Mukta M Webber, personal communication |
| 细胞说明书 | 登录后下载 |
| 中文名称 | 人正常前列腺基质细胞（永生化）（活细胞顺丰包邮，或可供冻存管） |
|
价格 |
1600 |
电话：021-54920405
传真：021-54920406
联系人：吴军
E-mail：ecell@sibs.ac.cn
地址：中国科学院细胞库，徐汇区岳阳路320号，上海200031
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传真：021-54921358
联系人：顾老师
E-mail：scell@sibcb.ac.cn
地址：中国科学院细胞库，徐汇区岳阳路320号，上海200031
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