细胞名称 Cell Name |
WPMY-1 |
目录号 Serial |
GNHu36 |
标识符 Identifier |
CSTR:19375.09.3101HUMGNHu36 |
描述 Description |
WPMY-1于1992年从一名54岁白人男性供者的基质中分离出来是一种肌成纤维基质细胞系, WPMY-1, 与RWPE-1 cells 一样,来源于同一张成人前列腺组织切片的周围区域的基质细胞。 通过一个pRSTV质料结构,用SV40大T抗原对基质细胞进行永生化。 WPMY-1细胞,与RWPE-1细胞及其它上皮细胞衍生株一样,属于来源于同一个前列腺的一系列细胞株。 由于它们来源于同一个前列腺的周围区域,WPMY-1细胞株对于研究分泌和基质与上皮细胞相互作用尤其有用。据提供者报道,来源于同一个前列腺的RWPE-1细胞株,经过检测,乙肝、丙肝、人免疫缺陷病毒都呈阴性。 细胞在本库通过支原体检测阴性(可供检测报告)。 细菌检测结果:阴性;真菌检测结果:阴性 细胞在本库通过STR检测无误(可供检测报告)。 STR鉴定结果: Amelogenin: X,Y D5S818:12,15 D13S317: 8,14 D7S820: 10,11 D16S539: 9 vWA: 14,18 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 13 D19S433: 13 D21S11: 29,31 D18S51: 14,16 |
动物种别 Organism |
人 白人 |
性别 Gender |
男性 |
组织来源 Tissue and Cell Type |
器官: 前列腺组织: 基质疾病: 正常 |
形态 Morphology |
成肌纤维细胞 贴壁生长 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
人正常前列腺基质永生化细胞WPMY-1完全培养液(目录号MD68)组分: DMEM培养基(GIBCO货号:10566016),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 培养参考:细胞消化时间2-3min。传代比例1:2,传代周期2-3天 冻存液配方:完全培养液92%,DMSO 8% 细胞运输方式:活细胞可保温装置运输或干冰冻存管。 注意事项:WPMY-1细胞生长较快,培养时细胞密度不能过高,70-80%即可传代。 |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
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细胞说明书 | 登录后下载 |
中文名称 | 人正常前列腺基质细胞(永生化)(活细胞顺丰包邮,或可供冻存管) |
价格 |
1600 |
电话:021-54920405
传真:021-54920406
联系人:吴军
E-mail:ecell@sibs.ac.cn
地址:中国科学院细胞库,徐汇区岳阳路320号,上海200031
电话:021-54921358
传真:021-54921358
联系人:顾老师
E-mail:scell@sibcb.ac.cn
地址:中国科学院细胞库,徐汇区岳阳路320号,上海200031
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