| 细胞名称 Cell Name |
HT-29 |
| 目录号 Serial |
TCHu103 |
| 标识符 Identifier |
CSTR:19375.09.3101HUMTCHu103 |
| 描述 Description |
HT-29是一种具有上皮形态的细胞系，于1964年从一名44岁白人女性结直肠癌患者的原发肿瘤中分离出来。该细胞系是一种适宜的转染宿主，在肿瘤和毒理学研究中具有应用价值。 用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离而来。近年来，已建株的培养细胞用含相同血清的McCOY's 5A培养液进行培养。该细胞系在裸鼠中致瘤，也能在类固醇处理的地鼠中致瘤。该细胞可合成IgA、CEA、TGFβ结合蛋白和黏液素，同时呈现出癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性；p53基因过表达，并且在p53基因的第273位密码子处发生G突变。细胞引进入库精准代数为P128代。 细胞在本库通过支原体检测阴性（可供检测报告）。 细菌检测结果：阴性；真菌检测结果：阴性 细胞在本库通过STR检测无误（可供检测报告）。 STR鉴定结果： Amelogenin: X D5S818:11,12 D13S317: 11,12 D7S820: 10 D16S539: 11,12 vWA: 17,19 TH01: 6,9 TPOX: 8,9 CSF1PO: 11,12 D19S433: 14 D21S11: 29,30 D18S51: 13 |
| 动物种别 Organism |
人 白人 |
| 性别 Gender |
女性 |
| 组织来源 Tissue and Cell Type |
结直肠腺癌 |
| 形态 Morphology |
上皮细胞，贴壁生长 |
| 培养基和添加剂 Complete Growth Medium and Culture Conditions |
人结肠癌细胞细胞HT-29完全培养液（目录号：MD120）组分： McCOY's 5A (Gibco货号：16600082)，90%；优质胎牛血清（GIBCO），10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 培养参考：细胞消化时间8-10min（0.25%胰酶）。传代比例1:2，传代周期2-3天 冻存液配方：完全培养液92%，DMSO 8% 细胞运输方式：活细胞可保温运输或干冰冻存管。 注意事项： HT-29细胞呈块片状聚集生长，细胞胞体黑点较多是正常现象。 |
| 供应限制 Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE | Journal of Interferon & Cytokine Research 24:219,2004. Didier ES , et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 editors. Human tumor cells in vitro. New York: Plenum Press; 1975, pp. 115-159. Chen TR , et al. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet. Cytogenet. 27: 125-134, 1987. PubMed: 3472642 Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Adachi A , et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832 Fantini J , et al. Human colon epithelial cells productively infected with human immunodeficiency virus show impaired differentiation and altered secretion. J. Virol. 66: 580-585, 1992. PubMed: 1727501 Butzow R , et al. A 60-kD protein mediates the binding of transforming growth factor-beta to cell surface and extracellular matrix proteoglycans. J. Cell Biol. 122: 721-727, 1993. PubMed: 8335695 Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Hanski C , et al. Tumorigenicity, mucin production and AM-3 epitope expression in clones selected from the HT-29 colon carcinoma cell line. Int. J. Cancer 50: 924-929, 1992. PubMed: 1372882 Reiter LS , et al. The role of the urokinase receptor in extracellular matrix degradation by HT29 human colon carcinoma cells. Int. J. Cancer 53: 444-450, 1993. PubMed: 8381394 Barnett SW , et al. Characterization of human immunodeficiency virus type 1 strains recovered from the bowel of infected individuals. Virology 182: 802-809, 1991. PubMed: 2024498 Shabahang M , et al. 1,25-Dihydroxyvitamin D3 receptor as a marker of human colon carcinoma cell line differentiation and growth inhibition. Cancer Res. 53: 3712-3718, 1993. PubMed: 8393379 Lesuffleur T , et al. Differential expression of the human mucin genes MUC1 to MUC5 in relation to growth and differentiation of different mucus-secreting HT- 29 cell subpopulations. J. Cell Sci. 106: 771-778, 1993. PubMed: 8308060 Pollack MS , et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Fantini J , et al. Infection of colonic epithelial cell lines by type 1 human immunodeficiency virus is associated with cell surface expression of galactosylceramide, a potential alternative gp120 receptor. Proc. Natl. Acad. Sci. USA 90: 2700-2704, 1993. PubMed: 8464878 Devedjian JC , et al. Regulation of the alpha 2A-adrenergic receptor in the HT29 cell line. Effects of insulin and growth factors. J. Biol. Chem. 266: 14359-14366, 1991. PubMed: 1677644 Santoro IM , Groden J . Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479 Bermudez LE , et al. Exposure to low oxygen tension and increased osmolarity enhance the ability of Mycobacterium avium to enter intestinal epithelial (HT-29) cells. Infect. Immun. 65: 3768-3773, 1997. PubMed: 9284150 Tsao H , et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 Qian XC , Brent TP . Methylation hot spots in the 5' flanking region denote silencing of the O6-methylguanine-DNA methyltransferase gene. Cancer Res. 57: 3672-3677, 1997. PubMed: 9288770 Morin PJ , et al. Apoptosis and APC in colorectal tumorigenesis. Proc. Natl. Acad. Sci. USA 93: 7950-7954, 1996. PubMed: 8755583 White LJ , et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293 Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Wang R , et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887 Young SW , et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865 Groh V , et al. Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium. Proc. Natl. Acad. Sci. USA 93: 12445-12450, 1996. PubMed: 8901601 Takahashi K , et al. Keratan sulfate modification of CD44 modulates adhesion to hyaluronate. J. Biol. Chem. 271: 9490-9496, 1996. PubMed: 8621620 |
| 细胞说明书 | 登录后下载 |
| 中文名称 | 人结肠癌细胞（活细胞顺丰包邮, 或可供冻存管） |
|
价格 |
1200 |
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E-mail：scell@sibcb.ac.cn
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